Amorphical evaluated the effect of different ACC concentrations on the rates of embryo
compaction at 60 h, blastocyst rate at 84 h, and percentage of fully hatched at 108 h
following hCG injection. As ACC is stabilized by tripolyphosphate (TPP), we also evaluated
the addition of TPP alone to the culture media. Finally, we compared supplemented ACC
culture media to one-step SAGE and Irvine cleavage media.
The results revealed that ACC accelerates the compaction and blastocyst rates and the
percentage of fully hatched embryos in a dose-dependent manner, with an increased
positive effect at 2.5 mM. The magnitude of the effect of ACC-supplemented media on the
embryo developmental rate was between 30 to 40% (p < 0.01) faster for each stage,
compared to both SAGE and Irvine one-step standard media. Embryos cultured with SAGE or
Irvine media, with or without supplementation of CaCl2 or CCC, did not produce the same
improvements as observed with ACC.
Conclusion In conclusion, the ACC demonstrates a rapid modulation effect for restoring
media optimal pH. ACC can inhibit cathepsin B activity during in vitro culture of fibroblast
cells. The beneficial impact of ACC on cleavage mouse embryos is likely due to an improved
buffering effect causing slower pH media variations, which may enhance the quality and
implantation potential of embryos following in vitro culture.
“The efect of amorphous calcium carbonate as a culture media supplement
on embryonic development of murine sibling embryos“
Amir Arav · Yehudit Natan · Tamila Hejja · Yigal D Blum · Ylena Poliansky · Pasquale Patrizio